ANXA6 Knockout cell line (THP-1)

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ANXA6 Knockout cell line (THP-1) Order

ANXA6 Knockout cell line (THP-1)
Catalog#
YKO-HT1219
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ANXA6 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ANXA6 Knockout cell line (THP-1)
Species
Human
Gene Name
ANXA6
Gene ID
309
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript ANXA6-201)
Validation: PCR+Sanger Sequencing
ANXA6 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANXA6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANXA6 gene knockout ANXA6 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ANXA6 Knockout cell line (THP-1)
ANXA6 Knockout cell line (THP-1)
Catalog#
YKO-HT1219
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ANXA6 Knockout cell line (Hep G2)

    ANXA6 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HT5169
    YKO-HT5169(Hep G2)
  • ANXA6 Knockout cell line (HEK293)

    ANXA6 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-H739
    YKO-H739(HEK293)
  • ANXA6 Knockout cell line (HeLa)

    ANXA6 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HS09261
    YKO-HS09261(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ANXA6 Knockout cell line (Hep G2)

    ANXA6 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HT5169
      inquiry   More details
  • ANXA6 Knockout cell line (HEK293)

    ANXA6 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-H739
      inquiry   More details
  • ANXA6 Knockout cell line (HeLa)

    ANXA6 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HS09261
      inquiry   More details

ANXA6 Knockout cell line (THP-1) Order

ANXA6 Knockout cell line (THP-1)
Catalog#
YKO-HT1219
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ANXA6 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ANXA6 Knockout cell line (THP-1)
Species
Human
Gene Name
ANXA6
Gene ID
309
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript ANXA6-201)
Validation: PCR+Sanger Sequencing
ANXA6 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANXA6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANXA6 gene knockout ANXA6 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ANXA6 Knockout cell line (THP-1)
ANXA6 Knockout cell line (THP-1)
Catalog#
YKO-HT1219
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ANXA6 Knockout cell line (Hep G2)

    ANXA6 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HT5169
    YKO-HT5169(Hep G2)
  • ANXA6 Knockout cell line (HEK293)

    ANXA6 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-H739
    YKO-H739(HEK293)
  • ANXA6 Knockout cell line (HeLa)

    ANXA6 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HS09261
    YKO-HS09261(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ANXA6 Knockout cell line (Hep G2)

    ANXA6 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HT5169
      inquiry   More details
  • ANXA6 Knockout cell line (HEK293)

    ANXA6 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-H739
      inquiry   More details
  • ANXA6 Knockout cell line (HeLa)

    ANXA6 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ANXA6
    Gene ID: 309
    Size: 1*10^6
    Catalog#: YKO-HS09261
      inquiry   More details

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